The most sets of twins in the same academic year at one school is 16 pairs and is shared by three schools. There are 16 sets of twins who were all enrolled in grade 9 at Valley Southwoods Freshman High School in West Des Moines, Iowa, USA, in the academic year 2011-2012. There are also 16 sets of twins who were all enrolled in grade 10 at Staples High School in Westport, Connecticut, USA, in the academic year 2011-2012 and 16 sets of twins in the graduating class of 2014 at the Maine South High School in Park Ridge, Illinois, USA.

The 2014 graduating class of Maine South High School also had 3 sets of triplets, qualifying it for the Most multiple birth sets in the same academic year at one school, as verified by Guinness World Records on 18 July 2011.