The most magicians in a magic show is 106 and was achieved by Magic Circle Germany and Julius Frack (Germany) at the Landestheater Tübingen, Germany, on 4 March 2012

The show started at 18:15 pm and ended at 21:15 pm, total duration 3 hours.
There was a 15 minute break which I did not deducted from the total duration (3 hours)